Cultured neuron expressing green fluorescent protein (GFP).

Adult brain lateral ventricle immunostained for Sox2 (red) and GFAP (green). Cell nuclei in blue.

Embryonic day 16 mouse brain cortex showing precursors along the lateral ventricle (red, Pax6), and neurons in the upper cortex (blue, SatB2). Cells that were previously transfected along the lateral ventricle with green fluorescent protein (green, GFP) alongside an unmarked knockdown vector have proliferated and migrated but remain stuck in the intermediate zone of the cortex.

Cultured cortical precursor cells after 7 days of treatment with medulloblastoma cell line conditioned media. GFAP (red) identifies the astrocytes while cell nuclei are in blue.

Precursor cells along the lateral ventricle were transfected with green fluorescent protein (GFP) at embryonic day (E13) and have divided, migrated radially and differentiated in the E16 mouse brain cortex.

7 day old cortical precursor cell culture in which a green fluorescent protein was expressed (green) to trace progeny of the precursor cells. Neurons (in red) are stained for Tuj1.

Mouse cortical cell culture: GFAP in green, Sox2 in magenta, Olig2 in blue.

GFP-expressing cultured neuron (green), and surrounding cells’ nuclei (blue).

Immunostaining of the perinatal day 9 (P9) brain sub-ventricular zone (SVZ) with Sox2 (magenta) and GFAP (white).

Cultured nerve-derived mesenchymal precursor cells, genetically tagged with the neural crest lineage tracer Wnt1Cre;TdTomato (red) and immunostained for the mesenchymal markers alpha smooth muscle actin (white) and Sox 9 (green).

The uninjured adult mouse digit tip contains Schwann cells that play a critical role in tissue regeneration, which are labeled by Sox2-CreERT2 lineage tracing (red) and further immunostained for P75NTR (green) to mark the Schwann cell lineage and their associated peripheral nerve axons.

An amputated adult mouse digit tip shown mid-regeneration revealing Wnt1-Cre lineage traced neural crest progeny (red) including peripheral nerves and nerve-derived mesenchymal precursor cells which contribute to regeneration.

Neural stem cells in the adult subventricular zone (SVZ) niche: the dorsolateral corner of the lateral ventricle, Sox2+ cells in red, GFAP+ cells in green.

An uninjured digit tip of Msx1CreERT2;R26R-LSL-TdT mouse immunostained for CD31 positive vasculature (green), TdTomato positive Msx1 lineage cells (red) and cell nuclei (white).

Mobile image of cultured neurons taken through the eyepiece of the microscope.

Cultured mesenchymal precursor cells dissociated from sciatic nerves, immunostained for the mesenchymal lineage marker alpha smooth muscle actin.

A digit tip of Msx1CreERT2;R26R-LSL-TdT mouse 14 days after digit amputation immunostained for CD31 positive vasculature (green), TdTomato positive Msx1 lineage cells (red) and cell nuclei (white).

Cultured neuron expressing green fluorescent protein.

An uninjured adult mouse digit tip of a 10kb-Dmp1-Cre-ERT2;R26R-LSL-TdT mouse. TdTomato positive Dmp1 lineage cells (red) and cell nuclei (white).

Immunostaining of the perinatal day 8 (P8) brain subventricular zone (SVZ) with Sox2 (green) and GFAP (magenta).

Embryonic day 16 mouse whole brain, showing green fluorescent protein expression in the cortex when exposed to the appropriate wavelength of light.

Cultured neurons expressing green fluorescent protein (green) and adjacent cells’ nuclei (blue), optically sectioned with the Apotome.

Mobile phone image of cultured neurons taken through the eyepiece of the microscope.

Sectioning embryonic mouse brains at -20C on the cryostat, prior to immunostaining and analysis.

Purified and cultured superior cervical ganglion neurons expressing green fluorescent protein.