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Cell nuclei in blue. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839395048-YGUG1IS2KIH95F2ICZWN/gallery5-e1520625599890.jpg Science Image Gallery - Photo Credit: Sarah Burns Embryonic day 16 mouse brain cortex showing precursors along the lateral ventricle (red, Pax6), and neurons in the upper cortex (blue, SatB2). Cells that were previously transfected along the lateral ventricle with green fluorescent protein (green, GFP) alongside an unmarked knockdown vector have proliferated and migrated but remain stuck in the intermediate zone of the cortex. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839391537-VFJS1PQMBE7JD18DL022/AlexGont.jpg Science Image Gallery - Photo Credit: Alexander Gont Cultured cortical precursor cells after 7 days of treatment with medulloblastoma cell line conditioned media. GFAP (red) identifies the astrocytes while cell nuclei are in blue. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839394399-HT9D60UGZTRCFQRGC9B3/gallery3-e1520625650955.jpg Science Image Gallery - Photo Credit: Miller Kaplan Lab https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839395439-SBHUVBY528YKYW3OIX28/gfp-brain-sized.jpg Science Image Gallery - Photo Credit: Sarah Burns Precursor cells along the lateral ventricle were transfected with green fluorescent protein (GFP) at embryonic day (E13) and have divided, migrated radially and differentiated in the E16 mouse brain cortex. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839392944-P68A1AQFLBZ69B1L3VOL/alexneuron.jpg Science Image Gallery - Photo Credit: Alexander Gont 7 day old cortical precursor cell culture in which a green fluorescent protein was expressed (green) to trace progeny of the precursor cells. Neurons (in red) are stained for Tuj1. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839393822-67SYCCH5IHB0RJRE5G0S/beatrixscience.jpg Science Image Gallery - Photo Credit: Beatrix Wang Mouse cortical cell culture: GFAP in green, Sox2 in magenta, Olig2 in blue. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839393703-39CXLMIPBZNXOLJ0FH0H/gallery1-e1520625698542.jpg Science Image Gallery - Photo Credit: Miller Kaplan Lab https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839394822-SWE2O7PYY3954TL4CGG0/gallery4-e1520625629428.jpg Science Image Gallery - Photo Credit: Sarah Burns GFP-expressing cultured neuron (green), and surrounding cells’ nuclei (blue). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839400683-RELBUU49J1T4BQMDZXFJ/Snapshot-of-MS94_P9_IL6_Sox2_GFAP_Ki67_X40_Image-4.jpg Science Image Gallery - Photo Credit: Mekayla Storer Immunostaining of the perinatal day 9 (P9) brain sub-ventricular zone (SVZ) with Sox2 (magenta) and GFAP (white). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839396101-RAI9U21USJ1MWRWY1LYM/Matt2.jpg Science Image Gallery - Photo Credit: Matthew Carr Cultured nerve-derived mesenchymal precursor cells, genetically tagged with the neural crest lineage tracer Wnt1Cre;TdTomato (red) and immunostained for the mesenchymal markers alpha smooth muscle actin (white) and Sox 9 (green). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839396578-A2JBSXGKPRU0T1F6B8ZJ/mattpic1.jpg Science Image Gallery - Photo Credit: Matthew Carr The uninjured adult mouse digit tip contains Schwann cells that play a critical role in tissue regeneration, which are labeled by Sox2-CreERT2 lineage tracing (red) and further immunostained for P75NTR (green) to mark the Schwann cell lineage and their associated peripheral nerve axons. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839396757-AA9XNK9DIYWE61RV4ZVX/mattpic2.jpg Science Image Gallery - Photo Credit: Matthew Carr and Patrick Steadman An amputated adult mouse digit tip shown mid-regeneration revealing Wnt1-Cre lineage traced neural crest progeny (red) including peripheral nerves and nerve-derived mesenchymal precursor cells which contribute to regeneration. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839397206-UZR83IKLRXK1DMW0F87K/MB-RMS.jpg Science Image Gallery - Photo Credit: Michael Borrett Neural stem cells in the adult subventricular zone (SVZ) niche: the dorsolateral corner of the lateral ventricle, Sox2+ cells in red, GFAP+ cells in green. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839397592-YSMXGCBUXM3ID20Q97VF/Picture-3.jpg Science Image Gallery - Photo Credit: Neemat Mahmud An uninjured digit tip of Msx1CreERT2;R26R-LSL-TdT mouse immunostained for CD31 positive vasculature (green), TdTomato positive Msx1 lineage cells (red) and cell nuclei (white). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839399057-7TJ3Z3XTWWUOD91DP5HE/sarahb2.jpg Science Image Gallery - Photo Credit: Sarah Burns Mobile image of cultured neurons taken through the eyepiece of the microscope. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839400933-CM9R2OPD1WA91N1F6RJR/wnt1.jpg Science Image Gallery - Photo Credit: Matthew Carr Cultured mesenchymal precursor cells dissociated from sciatic nerves, immunostained for the mesenchymal lineage marker alpha smooth muscle actin. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839398132-1HI26WW0J79LI9XTF5LJ/Picture-4.jpg Science Image Gallery - Photo Credit: Neemat Mahmud A digit tip of Msx1CreERT2;R26R-LSL-TdT mouse 14 days after digit amputation immunostained for CD31 positive vasculature (green), TdTomato positive Msx1 lineage cells (red) and cell nuclei (white). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839399571-TYNDUEBZU2BCJSVR69M5/scottpic.jpg Science Image Gallery - Photo Credit: Scott Yuzwa Cultured neuron expressing green fluorescent protein. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839398428-7KL31SYFI9Y86MQXA0L8/Picture-5.jpg Science Image Gallery - Photo Credit: Neemat Mahmud An uninjured adult mouse digit tip of a 10kb-Dmp1-Cre-ERT2;R26R-LSL-TdT mouse. TdTomato positive Dmp1 lineage cells (red) and cell nuclei (white). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839400417-LTHU7TC3QV4EJRKDRBJX/Snapshot-of-MS40_P8_IL6RFL_Cre_Sox2_GFAP_Ki67_X40_Image-3.jpg Science Image Gallery - Photo Credit: Mekayla Storer Immunostaining of the perinatal day 8 (P8) brain subventricular zone (SVZ) with Sox2 (green) and GFAP (magenta). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839398694-1NE5ENZM16KSKV5S61TY/sarahb1.jpg Science Image Gallery - Photo Credit: Sarah Burns Embryonic day 16 mouse whole brain, showing green fluorescent protein expression in the cortex when exposed to the appropriate wavelength of light. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839400135-ILS85QH8KD4WNDK4CFGV/Snap-108-ApoTome-49-e1520876224480.jpg Science Image Gallery - Photo Credit: Sarah Burns Cultured neurons expressing green fluorescent protein (green) and adjacent cells’ nuclei (blue), optically sectioned with the Apotome. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839399414-JZIOXD9Y370WBPND5MPQ/sarahb3.jpg Science Image Gallery - 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Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.millerkaplanlab.com/current-lab-members daily 0.75 2025-10-18 https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629909034762-RFG62BZ6Y90SJN8MJ40W/Headshot+of+Alice+Shan Current Members - Alice Shan, BSc MSc Candidate, Toronto Research Interests | Determination of neuroblastoma metastasis regulation Undergraduate | University of Toronto, Canada | Human Biology and Psychology https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1631037828732-UDTVII2VYGN4FX7KVLGI/Headshot+of+Jasmine+Yang Current Members - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1634057506575-D9JXKH9XA1O5F8HSA0K3/Headshot+of+Sarah+Ebert Current Members - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/8f2a0346-19c8-4ef7-9d33-8a2de0ea1b07/Retreat.jpeg Current Members - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1634056529050-HQDNL8AIXAKMO1RPZGWM/Headshot+of+Sandeep+Saxena Current Members - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1634060488931-ZL4QLTP05OGL74CDP4JB/Headshot+of+Ashleigh+Willis Current Members - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629908376890-IRO1YLAFWLZMR6QS0ELS/Headshot+of+Sruthi+Purushothaman Current Members - Sruthi Purushothaman, PhD Post Doctoral Fellow, Vancouver Research Interests | Digit tip development and regeneration. Doctorate | University of Kentucky, USA | Biology, Dr. Ashley W. Seifert | Characterization and functional analysis of patterning genes involved in axolotl limb development and its evolutionary perspective Graduate Degree | Cochin University of Science and Technology, India | Biotechnology, Dr. M. Mohammed Idris | Proteomic and gene expression analysis of zebrafish brain undergoing continuous light/dark stress Undergraduate | St.Xavier’s College for Women, India | Zoology https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629909348330-H5YSQ7VNJ4X4VKK01JSJ/beatrix.jpg Current Members - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629908197966-845LXISHU1S1QKYTQIG5/Headshot+of+Marissa+Lithopoulos Current Members - Marissa Lithopoulos, PhD Post Doctoral Fellow, Vancouver Research Interests | Relationships between neural stem cells and the vascular niche in the developing brain Doctorate | University of Ottawa, Canada | Cellular and Molecular Medicine, Dr. Bernard Thebaud | Utilizing umbilical cord mesenchymal stromal cell therapy to mitigate neural progenitor cell impairments in neonatal chronic lung injury models Undergraduate | University of Ottawa, Canada | Medical Science, minor in Philosophy https://www.millerkaplanlab.com/2021 daily 0.75 2025-05-09 https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/a6cf1ee6-348c-48ad-9eaf-4f1f744d03b7/2021-2.png 2021 - 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Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629835635682-OPQGP8M5X9DNA05IO2DO/2013-7.jpg Previous - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629822089831-KUZFWQ4G1BHRCIK6B04P/2017-5.jpg Previous - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629835572133-KTEUG9WZKJSRGFVRKGHK/2013-6.jpg Previous - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629822731082-KQP2608U8PJB0VAR9T35/2017other-2.jpg Previous - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.millerkaplanlab.com/lab-life daily 0.75 2025-05-09 https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1639082528516-9NCE2F9FBRXOCN9H16UV/image-asset.jpeg Lab Life - 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Christmas Party 2018 https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1630011123005-9ZKWPNLN6MG5JJRT8DHP/IMG_0001.jpg Lab Life - Sarah's 10th Anniversary - 2018 https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1630011144112-8RGTP1SJ71MCUQDVKS4O/IMG_2353.jpg Lab Life - Karaoke 2018 (Visit from Gianluca) https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1630011140557-MCTFZI80Q5X3XYNO7QZB/IMG_0526.jpg Lab Life - Island picnic - Summer 2018 https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1630011143743-DWMM48YE40DX6YP8QQVT/IMG_2350.jpg Lab Life - Karaoke 2018 (Visit from Gianluca) https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1630011137668-NII0U8B4Y2Q777B5PVQB/IMG_0522.jpg Lab Life - Kickball in the park - Summer 2018 https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1630011127775-D65E8IYUZIGVGHGB21X1/IMG_1.jpg Lab Life - Kickball in the park - Summer 2018 https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1630011138174-8L6989IV1WNGL7VRXSMJ/IMG_0523.jpg Lab Life - 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Photo Credit: Alexander Gont Adult brain lateral ventricle immunostained for Sox2 (red) and GFAP (green). Cell nuclei in blue. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839395048-YGUG1IS2KIH95F2ICZWN/gallery5-e1520625599890.jpg Science Images - Photo Credit: Sarah Burns Embryonic day 16 mouse brain cortex showing precursors along the lateral ventricle (red, Pax6), and neurons in the upper cortex (blue, SatB2). Cells that were previously transfected along the lateral ventricle with green fluorescent protein (green, GFP) alongside an unmarked knockdown vector have proliferated and migrated but remain stuck in the intermediate zone of the cortex. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839391537-VFJS1PQMBE7JD18DL022/AlexGont.jpg Science Images - Photo Credit: Alexander Gont Cultured cortical precursor cells after 7 days of treatment with medulloblastoma cell line conditioned media. GFAP (red) identifies the astrocytes while cell nuclei are in blue. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839394399-HT9D60UGZTRCFQRGC9B3/gallery3-e1520625650955.jpg Science Images - Photo Credit: Miller Kaplan Lab https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839395439-SBHUVBY528YKYW3OIX28/gfp-brain-sized.jpg Science Images - Photo Credit: Sarah Burns Precursor cells along the lateral ventricle were transfected with green fluorescent protein (GFP) at embryonic day (E13) and have divided, migrated radially and differentiated in the E16 mouse brain cortex. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839392944-P68A1AQFLBZ69B1L3VOL/alexneuron.jpg Science Images - Photo Credit: Alexander Gont 7 day old cortical precursor cell culture in which a green fluorescent protein was expressed (green) to trace progeny of the precursor cells. Neurons (in red) are stained for Tuj1. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839393822-67SYCCH5IHB0RJRE5G0S/beatrixscience.jpg Science Images - Photo Credit: Beatrix Wang Mouse cortical cell culture: GFAP in green, Sox2 in magenta, Olig2 in blue. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839393703-39CXLMIPBZNXOLJ0FH0H/gallery1-e1520625698542.jpg Science Images - Photo Credit: Miller Kaplan Lab https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839394822-SWE2O7PYY3954TL4CGG0/gallery4-e1520625629428.jpg Science Images - Photo Credit: Sarah Burns GFP-expressing cultured neuron (green), and surrounding cells’ nuclei (blue). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839400683-RELBUU49J1T4BQMDZXFJ/Snapshot-of-MS94_P9_IL6_Sox2_GFAP_Ki67_X40_Image-4.jpg Science Images - Photo Credit: Mekayla Storer Immunostaining of the perinatal day 9 (P9) brain sub-ventricular zone (SVZ) with Sox2 (magenta) and GFAP (white). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839396101-RAI9U21USJ1MWRWY1LYM/Matt2.jpg Science Images - Photo Credit: Matthew Carr Cultured nerve-derived mesenchymal precursor cells, genetically tagged with the neural crest lineage tracer Wnt1Cre;TdTomato (red) and immunostained for the mesenchymal markers alpha smooth muscle actin (white) and Sox 9 (green). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839396578-A2JBSXGKPRU0T1F6B8ZJ/mattpic1.jpg Science Images - Photo Credit: Matthew Carr The uninjured adult mouse digit tip contains Schwann cells that play a critical role in tissue regeneration, which are labeled by Sox2-CreERT2 lineage tracing (red) and further immunostained for P75NTR (green) to mark the Schwann cell lineage and their associated peripheral nerve axons. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839396757-AA9XNK9DIYWE61RV4ZVX/mattpic2.jpg Science Images - Photo Credit: Matthew Carr and Patrick Steadman An amputated adult mouse digit tip shown mid-regeneration revealing Wnt1-Cre lineage traced neural crest progeny (red) including peripheral nerves and nerve-derived mesenchymal precursor cells which contribute to regeneration. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839397206-UZR83IKLRXK1DMW0F87K/MB-RMS.jpg Science Images - Photo Credit: Michael Borrett Neural stem cells in the adult subventricular zone (SVZ) niche: the dorsolateral corner of the lateral ventricle, Sox2+ cells in red, GFAP+ cells in green. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839397592-YSMXGCBUXM3ID20Q97VF/Picture-3.jpg Science Images - Photo Credit: Neemat Mahmud An uninjured digit tip of Msx1CreERT2;R26R-LSL-TdT mouse immunostained for CD31 positive vasculature (green), TdTomato positive Msx1 lineage cells (red) and cell nuclei (white). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839399057-7TJ3Z3XTWWUOD91DP5HE/sarahb2.jpg Science Images - Photo Credit: Sarah Burns Mobile image of cultured neurons taken through the eyepiece of the microscope. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839400933-CM9R2OPD1WA91N1F6RJR/wnt1.jpg Science Images - Photo Credit: Matthew Carr Cultured mesenchymal precursor cells dissociated from sciatic nerves, immunostained for the mesenchymal lineage marker alpha smooth muscle actin. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839398132-1HI26WW0J79LI9XTF5LJ/Picture-4.jpg Science Images - Photo Credit: Neemat Mahmud A digit tip of Msx1CreERT2;R26R-LSL-TdT mouse 14 days after digit amputation immunostained for CD31 positive vasculature (green), TdTomato positive Msx1 lineage cells (red) and cell nuclei (white). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839399571-TYNDUEBZU2BCJSVR69M5/scottpic.jpg Science Images - Photo Credit: Scott Yuzwa Cultured neuron expressing green fluorescent protein. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839398428-7KL31SYFI9Y86MQXA0L8/Picture-5.jpg Science Images - Photo Credit: Neemat Mahmud An uninjured adult mouse digit tip of a 10kb-Dmp1-Cre-ERT2;R26R-LSL-TdT mouse. TdTomato positive Dmp1 lineage cells (red) and cell nuclei (white). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839400417-LTHU7TC3QV4EJRKDRBJX/Snapshot-of-MS40_P8_IL6RFL_Cre_Sox2_GFAP_Ki67_X40_Image-3.jpg Science Images - Photo Credit: Mekayla Storer Immunostaining of the perinatal day 8 (P8) brain subventricular zone (SVZ) with Sox2 (green) and GFAP (magenta). https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839398694-1NE5ENZM16KSKV5S61TY/sarahb1.jpg Science Images - Photo Credit: Sarah Burns Embryonic day 16 mouse whole brain, showing green fluorescent protein expression in the cortex when exposed to the appropriate wavelength of light. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839400135-ILS85QH8KD4WNDK4CFGV/Snap-108-ApoTome-49-e1520876224480.jpg Science Images - Photo Credit: Sarah Burns Cultured neurons expressing green fluorescent protein (green) and adjacent cells’ nuclei (blue), optically sectioned with the Apotome. https://images.squarespace-cdn.com/content/v1/60919af972196660f1ea650e/1629839399414-JZIOXD9Y370WBPND5MPQ/sarahb3.jpg Science Images - 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